About this manual. This manual describes the operation of the ÄKTA™FPLC™ system. System description, system maintenance and trouble-shooting are also. filter. Sample pump. Buffer select valve (V6). Injection valve (V1). Reverse . Manual. Flowpath. B1 or B2. BufferValveB. Pump A. Pump B. Common inlet. A2. A1. About this manual This manual describes the operation of ÄKTA purifier: Evaluating the UV response Calculate the UV response ratios in the following.
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Before moving the system, disconnect all cables and tubing connected to peripheral components and liquid containers. Detailed information on the components can be found in their respective User Manuals and Puriffier.
Tighten or replace if required. If the pressure exceeds the set point, another regulator decreases the flow rate in order to reduce the pressure. Add your deviation to these to obtain the correct value.
If used, the Union Luer connector replaces the injection fill port in port 3 of the injection valve. Some packing lists are included in the boxes. Active above the PressLevel value. Put the open manuxl in a waste container.
Follow local regulations mnual instructions for safe operation and maintenance of the system. The volumetric accuracy and precision is that of the syringe. The result file from a run holds a complete record of the run, including method, system settings, curve data and run log. The Chromatogram window contains all the curves. Its accurate response coupled with high precision over a wide measuring range makes it ideal for use in all chromatography techniques, from reversed phase with very low conductivity eluents to hydrophobic interaction chromatography in high salt solutions.
Material Color Volume of 10 cm A1, B1 cm 2. The pH electrode is delivered with a transparent cover. Chemical stains and dust should be removed. For low-pressure columns, at high flow rates. Ensure that it is not broken or dry.
A mm long i. Run Data The Run Data pane displays the current values for selected run parameters.
Fraction marks and fraction numbers make it easy to identify fractions and peaks. To change default correction factors for a recipe see Section 8. Low eluent flow and noise as the piston moves 1 Replace or re-install the seal or membrane. Purifieer calculate the new delay volume see Section 8. Use a flow restrictor. Do not raise to upright position yet. Lift off the mixer to remove the protective foam, and then refit. Brief descriptions of the individual components are given in Section 8.
ÄKTApurifier User Guide – GE Healthcare Life Sciences
Tubing and connectors for systems and purifer. PressLevel is the maximum allowed pressure. Check the valves are operating correctly. Two techniques can be used for filling the sample loop; partial or complete filling. The method run is initiated in a series of pages in Run Setup in System Control. The subsequent Sections describe: If is not within the limit, replace or contact GE Healthcare. Do not turn them on yet!
This is a base with only one pKa. FR is mounted in the system at delivery.
ÄKTApurifier User Guide – GE Healthcare Life Sciences |
Checking the optical unit 1 Check that the correct UV cell is fitted: Also select which recipe to use and the desired pH. A new column with identical values appears directly after the selected column. When a new method is created and a recipe is selected, default correction factors will apply. Calculation of the delay volume: This will create a report containing the chromatogram and the questions on one page.
In case of an emergency situation, the system mains switch must always be easy to access, and the mains plug must always be easy to disconnect. Select the appropriate printer from the list and select Landscape.
Refer to the “Technical specifications” for fuse data. The table below lists the theoretical volumes of a number of components and tubing.
Frac has only one UniNet-1 socket.
Complete filling is achieved as follows: The use of fiber optics together maunal a unique flow cell design ensures a high signal-to-noise ratio with a minimal drift and refractive index effects.